Water: Long Term 2 Enhanced Surface Water Treatment Rule
Laboratory Certification for Analysis of Cryptosporidium under the Safe Drinking Water Act
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The purpose of laboratory oversight is to identify laboratories that can reliably measure the occurrence of Cryptosporidium in surface water using approved methods.
For the first round (2006-2012) of Cryptosporidium monitoring under the Long Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR), EPA’s Office of Ground Water and Drinking Water (OGWDW) assumed responsibility for the approval and ongoing oversight of the laboratories that supported Public Water Systems (PWSs). In preparation for the second round of LT2ESWTR monitoring scheduled to begin in April 2015, OGWDW’s Technical Support Center (TSC) is working with EPA Regions and States to integrate Cryptosporidium laboratory approval/oversight into existing State certification/accreditation programs. State oversight of Cryptosporidium laboratories is provided for in the LT2ESWTR and is consistent with the laboratory oversight approach for all other regulated analytes. EPA Cryptosporidium laboratory approval granted by TSC does not carry over to the second round of LT2ESWTR Cryptosporidium monitoring that begins in 2015.
- On this page
Laboratory Certification Process
Laboratories wishing to support the LT2ESWTR Cryptosporidium monitoring program during the second round will need to:
- Step 1: Send your request and laboratory contact information to the EPA Region in which your laboratory resides
- Step 2: Continue to successfully participate in two Cryptosporidium Proficiency Testing events per year for each approved method for which the laboratory seeks certification/accreditation.
- Step 3: Successfully pass an audit by a qualified Cryptosporidium Certification Officer from:
- a) a State Program;
- b) a third party supporting a State Certification Program; or
- c) an EPA Regional Program.
|Region||States in Region||Contact|
|1||CT, NH, MA, ME, RI, VT||Jefferies.Ann@epa.gov|
|2||NY, NJ, PR, VI||Ringel.Donna@epa.gov|
|3||DC, DE, MD, PA, VA, WV||Messer.Ed@epa.gov|
|4||AL, FL, GA, KY, MS, NC, SC, TN||Maycock.Marilyn@epa.gov|
|5||IL, IN, MI, MN, OH, WI||Lagunas.Frank@epa.gov|
|6||AR, LA, NM, OK, TX||Clark.Rayr@epa.gov|
|7||IA, KS, MO, NE||Klepikow.Regina@epa.gov|
|8||CO, MT, ND, SD, UT, WY||Tidd.Marcie@epa.gov|
|9||AZ, CA, HI, NV||Lincoff.Andy@epa.gov|
|10||AK, ID, OR, WA||Dodo.Gerald@epa.gov|
States interested in integrating Cryptosporidium laboratory approval into their certification/accreditation program should:
- Step 1: Contact their Regional Certification Program to indicate their interest and request for nominations for participation in TSC’s next Certification Officer training course.
- Step 2: Familiarize themselves with the Cryptosporidium chapter (Chapter 7) in Supplement 2 to the Fifth Edition of the Manual for the Certification of Laboratories Analyzing Drinking Water.
Carrie Miller, Cryptosporidium Laboratory Technical Liaison
U.S. Environmental Protection Agency
Office of Ground Water and Drinking Water, Technical Support Center
26 West Martin Luther King Dr. (MS-140)
Cincinnati, Ohio 45268
Fax number: (513) 569-7191
E-mail: Carrie Miller (email@example.com)
Laboratories supporting the LT2ESWTR Cryptosporidium monitoring program during the second round
- Cryptosporidium Laboratories Acceptable for Monitoring to Comply with the Long Term 2 Enhanced Surface Water Treatment Rule (PDF) (2 pp, 293K)
- EPA Method 1623.1, January 2012 (PDF) (83 pp, 1MB)
- EPA Method 1623, December 2005 (PDF) (75 pp, 1MB)
- EPA Method 1622, December 2005 (PDF) (75 pp, 1MB)
Federal Register Notices
- Federal Register Notice, Vol. 79, No. 8 January 13, 2014
- Federal Register Notice, Vol. 74, No. 36 February 25, 2009
- Federal Register Notice, Vol. 70, No. 106 June 3, 2005
- Federal Register Notice, Vol. 67, No. 42 March 4, 2002
- Overview of US EPA Cryptosporidium and Giardia Detection Methods (Video)
Run Time 10.7 minutes, EPA 815-V-14-001
This video provides an overview of the U.S. Environmental Protection Agency’s Cryptosporidium and Giardia Detection Methods.
- Cryptosporidium in Water: Reviewing Laboratory Reports (Video)
Run Time 2.5 minutes
This video describes key review elements to increase one's understanding of laboratory reports and quality control for Cryptosporidium analyses.
- Cryptosporidium Analysis: Using a Dispersant (Video)
Run Time 2.4 minutes
This video demonstrates the new dispersant addition procedure, detailed in the recently published Method 1623.1, that improves Cryptosporidium recovery from difficult matrices.
- Frequently Asked Questions: State Approval/Oversight of Cryptosporidium Laboratories Supporting LT2ESWTR Monitoring (PDF) (3 pp, 218K)
- Supplement 2 to the Fifth Edition of the Manual for the Certification of Laboratories Analyzing Drinking Water
Training ModulesThe training modules provide guidance and instruction to utility and laboratory personnel on LT2ESWTR sample collection and analysis.
- Adobe Flash Player is required to view the modules.
- Module Access Directions (PDF) (1 pg, 114 K)
- Sample Collection (56.7 MB ZIP File)
This module details the sampling procedures for bulk sample collection and field filtration, and provides instruction on proper sample packing and shipping.
- Sample Collection Pocket Guide (PDF) EPA 817-K-06-002 (6 pp, 1.1 MB)
- Order a hardcopy of the pocket guide through EPA’s National Service Center for Environmental Publications (NSCEP).
- Basic Microscopy (18.7 MB ZIP File)
This module assists analysts in the proper use and maintenance of a microscope as used to detect Cryptosporidium and Giardia in Method 1623.
- Microscopic Identification (13.4 MB ZIP File)
This module helps analysts properly identify and characterize Cryptosporidium and Giardia using Method 1623. The module includes characterization by FITC, DAPI, and DIC of positive control organisms and interfering organisms.
- Immunomagnetic Separation (25.6 MB ZIP File)
This module details the immunomagnetic separation (IMS) procedure used in EPA Method 1623 to separate Cryptosporidium oocysts and Giardia cysts from extraneous debris in a water sample.